Bacterial product.



UNITE STATES PATENT OFFICE.

WERNER SCHULTZ, OF GHARLOTTENBURG, GERMANY.

BACTERIAL PRODUCT.

nooaovv.

No Drawing.

To all whom it may concern:

Be it known that I, VVERNER SCHULTZ, a subject of the Emperor ofGermany, residing at Charlottenburg, near Berlin, Germany, have inventedcertain new and useful llmprovements in Bacterial Products; and I dohereby declare the following to be a full, clear, and exact descriptionof the invention, such as will enable others skilled in the art to whichit appertains to make and use the same.

The subject of my invention is a process of obtaining a liquidpreparation for diagnostic and curative purposes.

According to my invention the pure cultures of bacilli and a culturemedium are submitted to evaporation and to the concentrated liquid,after the mass of bacilli has been filtered ofi, there is added an ironcon1- pound, such for instance as iron oxychlorid. The precipitate thusobtained is washed till free from chlorin and albumin, and thendissolved in diluted soda lye.

A reaction takes place between the active principles of the extractformed by the evaporation and the iron salt. This tuberculin is asolution of the iron albumin obtained as a precipitate on adding an ironcompound to the culture medium which has been evaporated to its tenthpart. This method is particularly suitable for pre paring tuberculin andhas the advantage that the product'is essentially purer than tuberculinas hitherto prepared. By means of the present invention, on the otherhand, I obtain a preparation free from the said by- Specification ofLetters Patent.

Application filed January 18, 1910. Serial No. 538,666.

Patented Oct. 3, 1911.

oxychlorid which is added varying between 90 and 120 cubic centimeters.I-Iereupon the precipitate is washed till free from chlorin and albuminand dissolved in dilute soda lye of 1% at an amount of 30 to drops,adding such a quantity of a mixture of water and 25% glycerin that theresulting liquid amounts 40 cubic centimeters.

What I claim is- The process of obtaining a preparation for diagnosticand curative purposes, according to which the pure cultures areevaporated with the culture medium, whereupon the concentrated liquid isfiltered to remove the bacilli mass, and an iron compound added to it,after which the precipitate is washed, and dissolved in soda lye,substantially as described.

In testimony whereof I aifix my signature in presence of two witnesses.

WERNER SCHULTZ.

Witnesses HUGH GRAMATKI, LARAPED BEISHAN.

Copies of this patent may be obtained for five cents each, by addressingthe Commissioner of Patents, Washington, D. C.

